Effect of bone xenograft extract on biological characteristics of bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2015.36.003
- VernacularTitle:异种骨材料浸提液对骨髓间充质干细胞生物学特性的影响
- Author:
Fei LI
;
Yang CHEN
;
Zhanchang WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(36):5753-5757
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:How to preserve the biological properties and osteogenic activity of bone xenograft materials after antigen removal has become one of the hot spots. OBJECTIVE:To investigate the effect of bone xenograft extract on proliferation, migration and osteogenic differentiation of bone marrow mesenchymal stem cel s. METHODS:Bone marrow mesenchymal stem cel s from Sprague-Dawely rats were cultured respectively in bone xenograft extract (experimental group) and DMEM/F12 medium containing 10%fetal bovine serum (control group). MTT, Transwel chamber and flow cytometry were used to detect cel proliferative ability, migration ability and cel cycle changes. Passage 3 bone marrow mesenchymal stem cel s were selected in the two groups and cultured in osteogenic induction medium. At 7 and 14 days of osteogenic induction, alkaline phosphatase staining was done;at 21 days of osteogenic induction, alizarin red staining was performed to observe calcium nodule formation. RESULTS AND CONCLUSION:After 1, 3, 5, 7 days of culture, the cel proliferative activity and cel cycle had no significant difference between the two groups, but the number of migrating cel s was significantly lower in the control group than the experimental group (P<0.05). At 7 and 14 days of culture, the alkaline phosphatase level was significantly higher in the experimental group than the control group (P<0.05);at 21 days of culture, calcium nodes formed in both two groups, but the calcium nodule area was larger in the experimental group than the control group. These findings indicate that bone xenograft materials have no cytotoxicity but possess good cytocompatibility, which can promote the proliferation, migration and osteogenic differentiation of bone marrow mesenchymal stem cel s.
