Interventional effects of different Chinese medicine therapies on proliferation and differentiation of bone marrow mesenchymal stem cells homing to the border zone of myocardial infarction
10.3969/j.issn.2095-4344.2015.36.007
- VernacularTitle:不同治法中药干预心肌梗死边缘区归巢骨髓间充质干细胞的增殖与分化
- Author:
Jinsheng ZHANG
;
Baoxia ZHANG
;
Yangyang ZHANG
;
Huifang ZHU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(36):5774-5781
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:New ideas for regenerative treatment of cardiovascular disease are as fol ows:to understand the changes in al kinds of stem cel s differentiating into cardiomyocytes, to determine the physiological role of differentiated stem cel s in cardiac functional activities, to stimulate the proliferative potential of various stem cel s under certain conditions that can directly differentiate into functional cardiomyocytes, thereby replenishing deficient cardiomyocytes and effectively inhibiting excessive proliferation of fibroblasts. OBJECTIVE:To investigate the effects of panax notoginseng saponins, salidroside and astragalus effective components on the homing, proliferation and differentiation of endogenous CD105-positive bone marrow mesenchymal stem cel s of myocardial infarction rats. METHODS:Acute myocardial infarction rats were randomly divided into activating blood circulation group, tonifying qi group, activating blood circulation+tonifying qi group (combined group), model group. Normal rats served as control group. The former three groups were oral y given 80 g/L Xuesaitong soft capsules (panax notoginseng saponins as the main component), 0.5 g/mL astragalus particles and 70 g/L Nuodikang capsules (salidroside as the main component), respectively, at a dose of 1 mL/100 g, once a day, total y for 28 days. The control and model groups were given the same volume of normal saline. Expressions of CD105, CD117, vimentin, cardiac troponin T (cTnT), zinc finger transcription factor 4 (GATA-4) and Ki-67 were detected using immunohistochemical method at 1, 3, 7, 14, 28 days of drug administration. RESULTS AND CONCLUSION:(1) Compared with the control group, the expressions of CD105, CD117, cTnT, GATA-4 and Ki-67 were higher in the model group, which were increased at 1-7 days, peaked at 7 days, and then decreased. Compared with the model group, the expressions of CD105, CD117, cTnT, GATA-4 and Ki-67 were significantly higher in the activating blood circulation group, tonifying qi group and combined group, especial y in the activating blood circulating group, at different time of drug administration (P<0.05). In each group, the staining results of Ki-67 were not exactly paral el to those of CD105, CD117, cTnT and GATA-4, but their rising tendency was substantial y the same. (2) Compared with the control group, the vimentin expression in the model group was higher, which showed an increasing tend at 1-3 days, peaked at 3 days and then declined. Compared with the model group, the vimentin expression was significantly lower in the activating blood circulation group, tonifying qi group and combined group, especial y in the activating blood circulating group, at different time of drug administration (P<0.05). It suggested that the activating blood circulation group had a remarkable antifibrotic role. These findings indicate that panax notoginseng saponins, salidroside and astragalus effective components al can promote the proliferation and differentiation of bone marrow mesenchymal stem cel s to delay myocardial remodeling, but the recipe for promoting blood circulation has the most obvious outcomes.
