A static magnetic field with certain intensity can promote the secretion of nerve growth factor from Schwann cells
10.3969/j.issn.2095-4344.2015.36.020
- VernacularTitle:一定强度静磁场可促进许旺细胞快速分泌神经生长因子
- Author:
Lingyun WEI
;
Ruiying WANG
;
Jicun TANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(36):5848-5852
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:There are less studies on static magnetic field, and its biological effects are stil unclear. OBJECTIVE:To investigate the static magnetic field effect on the secretion of nerve growth factor from Schwann cel s. METHODS:Schwann cel s were randomly assigned into three groups:0.05 mT sciatic magnetic field group, 0.1 mT sciatic magnetic field group and control group. At 2 days of inoculation, the cel s were exposed to the sciatic magnetic field, 12 hours per day. Cel s in the control group were not exposed to the sciatic magnetic field. After 6 days of exposure, RT-PCR method was used to detect nerve growth factor mRNA expression in Schwann cel s, and ELISA was adopted to detect the level of nerve growth factor secreted from Schwann cel s. RESULTS AND CONCLUSION:The mRNA expression and secreted level of nerve growth factors in the culture supernatant were significantly higher in the two sciatic magnetic field groups than the control group (P<0.05), especial y in the 0.1 mT group. However, there was no difference between the 0.05 mT and 0.1 mT groups (P>0.05). These findings indicate that the static magnetic field with certain intensity can promote the secretion of nerve growth factors from Schwann cel s.
