Mechanism by which interferon reduces the resistance of MGMT positive glioma stem cells to temozolomide

VernacularTitle:干扰素抗MGMT阳性胶质瘤干细胞替莫唑胺的耐药机制
Author: Hui SU ; Zhaowei LIU ; Hongli DU ; Yanmei QIAO
Publication Type:Journal Article
From: Chinese Journal of Tissue Engineering Research 2015;(36):5800-5805
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To investigate the role of interferon to increase the sensibilization of MGMT positive glioma stem cel s to temozolomide in vitro. METHODS:Glioma cel lines, U251 and SKMG-4, were induced by suspended cloning bal formation method to harvest MGMT positive glioma stem cel s, U251G and SKMG-4G. Cel counting kit-8 assay was used to detect the kil ing effect of interferonα/βcombined with temozolomide on MGMT positive glioma stem cel s. RT-PCR and western blot assay were employed to determine the expression of MGMT and nuclear factorκB in MGMT positive glioma stem cel s. RESULTS AND CONCLUSION:Western blot results showed positive expression of MGMT in U251G and SKMG-4G cel s at protein levels. After intervention with interferonα/β, the mRNA expression of MGMT and nuclear factorκB in SKMG-4G and U251G cel s was reduced significantly, and then further decreased after temozolomide treatment. These findings indicate that interferonα/βcan remarkably strengthen the kil ing effect of temozolomide on MGMT positive glioma stem cel s.