Telomerase reverse transcriptase gene transfection of human amniotic electrical mesenchymal stem cell transplantation for treatment of diabetes mellitus
10.3969/j.issn.2095-4344.2015.36.016
- VernacularTitle:端粒酶反转录酶基因电转染人羊膜间充质干细胞移植治疗糖尿病
- Author:
Jianru FU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(36):5827-5832
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:The amniotic membrane is the rejected material after birth. Amniotic mesenchymal stem cel s are characterized as easy harvesting, strong proliferation ability, no ethical controversy, and low immunogenicity. OBJECTIVE:To electrotransfer human telomerase reverse transcriptase (hTERT) gene into amniotic mesenchymal stem cel s transplanted into diabetic rats and to explore its effect on diabetic rats. METHODS:Human amniotic mesenchymal stem cel s were isolated, cultured and electrotransferred by hTERT gene. Ten of 50 Sprague-Dawley rats were randomized selected as controls, and the remaining rats were used to establish diabetic models through injection of 45 mg/kg streptozotocin. Thirty-six model rats were randomized into model group, cel transplantation group and hTERT-transfected cel transplantation group, with 12 rats in each group. In the latter two groups, human amniotic mesenchymal stem cel s and hTERT-transfected amniotic mesenchymal stem cel s were injected via sublingual veins, respectively. After transplantation, blood glucose levels were monitored dynamical y, and plasma insulin concentration was detected every week. Pancreas tissues were taken and cut into sections for histological observation using hematoxylin-eosin staining. RESULTS AND CONCLUSION:At 4 weeks after transplantation, the blood glucose levels were significantly lower in the two cel transplantation groups than the model group (P<0.05), and especial y in the hTERT-transfected cel transplantation group, the blood glucose level was close to the normal value (P>0.05). However, the model group stil had a higher blood glucose level. At 6 weeks after transplantation, compared with the model group, the plasma insulin concentration was significantly increased in the two cel transplantation group (P<0.05), and the severity of pancreatic injury was also eased in these two groups (P<0.05), especial y in the hTERT-transfected cel transplantation group (P<0.05). These findings indicate that hTERT-transfected amniotic mesenchymal stem cel transplantation can dramatical y decrease blood glucose level and relieve pancreatic injury in diabetic rats, which is an effective method for treatment of mel itus diabetes in rats.
