Lung Infection Caused by Mycobacterium riyadhense Confused with Mycobacterium tuberculosis: The First Case in Korea.
10.3343/alm.2012.32.4.298
- Author:
Jung In CHOI
1
;
Ji Hun LIM
;
Sung Ryul KIM
;
Seon Ho LEE
;
Jae Sun PARK
;
Kwang Won SEO
;
Jae Bum JEON
;
Joseph JEONG
Author Information
1. Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea. 690519@hitel.net
- Publication Type:Case Reports
- Keywords:
Nontuberculous mycobacteria;
Mycobacterium;
Mycobacterium riyadhense;
Mycobacterium simulans
- MeSH:
Adult;
Antitubercular Agents/pharmacology;
Chromatography, High Pressure Liquid;
Female;
Humans;
Lung Diseases/*microbiology;
Microbial Sensitivity Tests;
Mycobacterium/classification/drug effects/*isolation & purification;
Mycobacterium Infections/microbiology;
Mycobacterium tuberculosis/genetics/isolation & purification;
Mycolic Acids/analysis;
Oligonucleotide Array Sequence Analysis;
Phylogeny;
RNA, Ribosomal, 16S/chemistry/genetics;
RNA, Ribosomal, 23S/chemistry/genetics;
Republic of Korea;
Sequence Analysis, DNA
- From:Annals of Laboratory Medicine
2012;32(4):298-303
- CountryRepublic of Korea
- Language:English
-
Abstract:
A slowly growing, non-chromogenic mycobacterial strain was isolated from sputum and bronchial lavage fluid samples of a patient presenting with productive cough, blood-tinged sputum, low-grade fever, and weakness. A positive acid-fast bacilli sputum smear result prompted the initiation of an anti-tuberculosis regimen. Multiplex real-time PCR showed a negative result for Mycobacterium tuberculosis complex and a positive result for nontuberculous mycobacteria. The DNA chip test confirmed this organism as a member of the genus Mycobacterium, but could not specify the species. Interestingly, the mycolic acid patterns obtained by HPLC nearly overlapped with those of M. simulans. The sequences of the Mycobacterium 16S rRNA gene and 16S-23S internal transcribed spacer region were unique and were found to have 100% similarity with those of M. riyadhense. After a review of the literature, we report this case as the first Korean case of M. riyadhense lung infection.
