Expression of octamer-binding transcription factor 4 in gastric cancer and its effects on gastric cancer cell proliferation and apoptosis

VernacularTitle:胚胎干细胞标志物转录因子在胃癌中的表达及对细胞增殖和凋亡的影响
Author: Yijiu SHI ; Jiangfeng QIU ; Lei FANG ; Huifang WANG ; Xiuchong YU
Publication Type:Journal Article
Keywords: Stomach neoplasms; RNA interference; Cell proliferation; Apoptosis
From: Chinese Journal of General Surgery 2015;30(7):553-555
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of transcription factor Oct 4 in gastric carcinoma and its effects on gastric cancer cell proliferation and apoptosis after Oct 4 gene interfered by lentivirus vector.Methods Real time PCR and Western blot were used to observe the expression of Oct 4 in different differentiated gastric cancer cell lines.Gastric cancer cell lines with high expression of Oct 4 was cultured and infected by siRNA-Oct 4-lentivirus vector.Cell proliferation and apoptosis were observed after Oct 4 gene was interfered.Results Oct 4 was highly expressed in poorly and moderately differentiated gastric cancer cells.Gene interfered with siRNA inhibits the expression of Oct 4 in gastric cancer cells and show significant effects on cell proliferation and mobility as well as apoptosis after down-regulation of Oct 4.Condusions Oct 4 expression is in close relationship with gastric cancer cell proliferation and invasive ability.