Role of NADPH oxidase in high glucose-induced injury in H9 c2 cardiac cells
10.3969/j.issn.1001-1978.2015.10.011
- VernacularTitle:NADPH氧化酶在高糖诱导的H9 c2心肌细胞损伤中的作用
- Author:
Wei YU
;
Qing MIN
;
Shuang GUO
- Publication Type:Journal Article
- Keywords:
myocardial cells;
high glucose;
diabetic cardiomyopathy;
NADPH oxidase;
oxidative stress;
ap-optosis
- From:
Chinese Pharmacological Bulletin
2015;(10):1379-1382
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the role of nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase in high glucose-induced injury in H9c2 cardiac cells. Methods H9 c2 cardiac cells were exposed to differ-ent concentrations of high glucose(5. 5 mmol·L-1 ,11 mmol · L-1 ,22 mmol · L-1 ,33 mmol · L-1 ,44 mmol ·L-1 , 55 mmol · L-1 ) for 24 h and different time pints of high glucose(0 h,12 h,24 h,36 h,48 h,72 h) . Cell viability was measured by MTT colorimetry, the protein expression of Bcl-2 , Bax and NADPH oxi-dase submits such as p22 phox , p47 phox and p67 phox were determined by western blotting. Results H9c2 cardi-ac cells exposure to high glucose for 24 h showed on decrease in cell survival and the Bcl-2 expression while an increase in the Bax expression ( P<0. 05 ) . Moreo-ver, high glucose could markedly up-regulate the activ-ity of NADPH oxidase characterized by the enhanced expression of p22 phox , p47 phox and p67 phox ( P<0. 05 ) . Conclusion Activating NADPH oxidase may play an important role in high glucose-induced injury in H9 c2 cells.
