Effect of 3 isoforms of Ikaros on proliferation of human ovarian cancer SKOV3 cells
10.3969/j.issn.1000-4718.2015.08.010
- VernacularTitle:Ikaros的3种亚型对人卵巢癌 SKOV3细胞增殖的影响
- Author:
Licai HE
;
Shang CHEN
;
Zhenfeng ZHU
;
Jun GAN
;
Huimin YU
- Publication Type:Journal Article
- Keywords:
Ikarosisoforms;
Ovariancancer;
Cellproliferation;
Cellcycle
- From:
Chinese Journal of Pathophysiology
2015;(8):1407-1411
- CountryChina
- Language:Chinese
-
Abstract:
AIM:ToinvestigatetheeffectofIkarosisoformsontheproliferationofhumanovariancancerSK-OV3 cells.METHODS:Three isoforms of Ikaros, IK1, IK2 and IK6, were transfected into ovarian cancer SKOV3 cells. CCK-8 assay and cell counting were used to detect the effects of Ikaros isoforms on the proliferation of SKOV 3 cells.The cell cycle was analyzed by flow cytometry .The cell cycle-related proteins were detected by Western blot .RESULTS:IK1 and IK2 expression inhibited SKOV 3 cells proliferation .Flow cytometry analysis indicated that IK 1 and IK2 induced SK-OV3 cell cycle arrest at the G 1 phase.IK6 isoform exerted no obvious effect on the proliferation or cell cycle of SKOV 3 cells.Compared with control EV group , IK1 group and IK2 group showed a dramatic elevation in the expression of the cell cycle inhibitor p21, along with a substantial decrease in the expression of the cell cycle inducers cyclin D 1 and cyclin D2, which did not change in IK 6 group.CONCLUSION:IK1 and IK2 significantly inhibit the proliferation of ovarian cancer SKOV3 cells and induce cell cycle arrest at G 1 phase by regulation of cell cycle-related proteins cyclin D1, cyclin D2 and p21, while IK6 isoform exerts no obvious effect on the proliferation and cell cycle of SKOV 3 cells.
