Effect of Furin Inhibitor on Growth of Breast Cancer M CF￣7 Cell
10.3870/j.issn.1004-0781.2015.11.007
- VernacularTitle:弗林蛋白转换酶抑制剂对乳腺癌 MCF￣7细胞增殖的影响
- Author:
Xiaojuan DU
;
Yunfeng LI
;
Yan WANG
;
Hongwei WANG
;
Xuejun LI
- Publication Type:Journal Article
- Keywords:
Furin inhibitor;
Cancer,breast;
Cell apoptosis;
Superoxide dismutase;
Catalase
- From:
Herald of Medicine
2015;(11):1444-1447
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Furin in breast cancer cell proliferation and provide a theoretical basis for in￣depth study of breast cancer. Methods Different concentrations of Furin inhibitor were added in MCF￣7 cell culture to test MCF￣7 cell proliferation by MTT essay.Hochest 33342 staining was used to detect the morphological change of apoptosic cells.Western blot analysis was applied to measure the level of cell apoptosis associated proteins,such as Caspase￣3,Caspase￣8 andCaspase￣9.The enzyme￣linked immunosorbent assay was used for detection the CAT and SOD levels in cell culture. ResultsMCF￣7 cell growth was inhibited by Furin inhibitor in a time and dose dependent manner.The results of Western blot and Hochest33342 staining indicated that MCF￣7 cells were apoptosis after Furin inhibitor treatment. The level of CAT was increasedsignificantly,associated with the level of SOD. Conclusion Furin inhibitor could induce MCF cell apoptosis, thereby inhibitcell proliferation by modulating MCF￣7 cell redox state.
