Study on Quality Standard of Huangdi Cataplasm
10.3870/j.issn.1004-0781.2015.11.023
- VernacularTitle:黄地巴布剂质量标准研究
- Author:
Junjie HU
;
Baohui ZHANG
;
Qiuxia LYU
;
Guihong WANG
;
Guohua ZHENG
- Publication Type:Journal Article
- Keywords:
Huangdi cataplasm;
Astragaloside A;
Loureirin B;
Chromatography,thin￣layer;
Chromatography,high performance liquid;
Quality standard
- From:
Herald of Medicine
2015;(11):1501-1505
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the quality standard for evaluating Huangdi cataplasm. Methods Thin layer chromatography (TLC) was used to qualitatively identify Astragalus membranaceus (Fisch.) Bunge,Rheum palmatum Linn,Rhizoma Chuanxiong,Angelica sinensis and Resina Draconis in Huangdi cataplasm.HPLC method was used to determine astragaloside A and loureirin B in Huangdi cataplasm. Results The Astragalus membranaceus (Fisch.) Bunge,Rheum palmatum Linn,Rhizoma Chuanxiong,Angelica sinensis and Resina Draconis were well separated by TLC without interference in the negative control.content of Astragaloside A and loureirin B showed good liner relationships with respective peak area within the range of 6.96-23.2 μg,and 0.072-0.648 μg,with r = 0.999 5,r = 0.999 9, respectively;and the average recovery was 97.18%,and 96.93%,RSD was 1.21%(n= 6),1.53% (n = 6 ), respectively. Conclusion The established qualitative and quantitative detection method is simple, specific, reproducible, accurate and reliable, which can be used for quality control of Huangdi cataplasm.
