Establishment of Fingerprint and Mutual Mode of Aqueous Extracts of Eucommia ulmoides Oliv.from Different Regions by HPLC

VernacularTitle:不同产地杜仲药材水提液高效液相色谱指纹图谱及其共有模式
Author: Xuying HUANG ; Ling CHEN ; Jing CHENG ; Hongping SONG
Publication Type:Journal Article
Keywords: Eucommia ulmoides Oliv.; Chromatography,high performance liquid; Fingerprint; Mutual mode
From: Herald of Medicine 2015;(11):1497-1500
CountryChina
Language:Chinese
Abstract: Objective To establish fingerprint and mutual mode of aqueous extracts of Eucommia ulmoides Oliv.from different regions by high performance liquid chromatography ( HPLC). Methods The column of SinoChrom ODS￣BP (250 mm×4.6 mm, 5 μm) was used.The mobile phase consisted of acetonitrile￣0.1% phosphoric acid with gradient elution.The flow rate was 1.0 mL.min-1 , the detective wavelength was 230 nm, and the column temperature was 25 ℃ . Results The fingerprint consisted of 14 common peaks.The overall similarity for ten batches of Eucommia ulmoides Oliv.from different regions was 0.592-0.986.The standard fingerprint of Eucommia ulmoides Oliv.was established by HPLC. Conclusion This fingerprint method is simple and repeatable, and it provides a scientific basis for the quality control of Eucommia ulmoides Oliv.