Target genes of the hsa-miR-106b-25 cluster:Prediction and bioinformatic analysis
10.16571/j.cnki.1008-8199.2015.10.004
- VernacularTitle:Hsa-miR-106b-25簇的靶基因预测及生物信息学分析
- Author:
Yaqin SONG
;
Wenbo FU
;
Mu LU
;
Yang LIU
;
Yutao WEI
- Publication Type:Journal Article
- Keywords:
has-miR-106-25 cluster;
miR-106b;
miR-93;
miR-25;
Carcinoma;
Bioinformatic analysis
- From:
Journal of Medical Postgraduates
2015;28(10):1023-1027
- CountryChina
- Language:Chinese
-
Abstract:
Objective The hsa-miR-106b-25 gene cluster is involved in various biological processes of carcinoma .This study aims at a prediction and function analysis of the hsa-miR-106b-25 gene cluster, miR-106b, miR-93, and miR-25, so as to provide some evidence for further studies on the functions of the three miRNAs and the mechanisms of their interaction . Methods We obtained the sequences of miR-106b, miR-93, and miR-25 from the miRBase, predicted their target genes with TargetScan , PicTar, and miRanda, and used 3 or more experimentally verified target genes from the miRTarbase as the gene set for further bioinformatic analysis .We predic-ted the biological processes of the target genes by GeneOntology analysis and enriched KEGG ( Kyoto Encyclopedia of Genes and Genomes) by pathway analysis, produced protein-protein interaction ( PPI) networks with STRING . Results The target genes of the miR-106b-25 gene cluster were significantly enriched in such biological processes as the regulation of macromolecule metabolism , regulation of metabolic process , and cell cycle process , while the KEGG pathway mainly in glioma, melanoma, prostate cancer , and gallbladder carcino-ma.The proteins encoded by the targeted genes of showed complicated interactions , and those encoded by the KAT2B, PTEN, TP53, CDH1, MDM2, E2F1, RB1, and SMAD7 plaid a core role in the interac-tion network. Conc lusoi n MiRNAs of the miR-106b-25 gene cluster regulate the downstream target proteins involved in tumorigenesis by participating in the cell cycle and cancer signaling pathway .
