Mechanism of apoptosis induced by juglone in human cervical cancer SiHa cells
10.3867/j.issn.1000-3002.2015.05.060
- VernacularTitle:胡桃醌诱导人宫颈鳞癌SiHa细胞凋亡机制
- Author:
Wei ZHANG
;
Wenhe ZHU
;
Yan LI
;
Jun LUO
;
Xingyu ZHAO
;
Junjie XU
;
Yanxia JIANG
;
Shijie LYU
- Publication Type:Journal Article
- Keywords:
juglone;
PI3K/AKT;
apoptosis;
SiHa cells
- From:
Chinese Journal of Pharmacology and Toxicology
2015;(5):831-835
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the pro-apoptotic mechanism of juglone in SiHa cells and to in?vestigate its associated signal transduction pathways. METHODS SiHa cells were treated with juglone 20μmol·L-1 for 24,48 and 72 h. Cellular morphology was detected by inverted microscopy.The cell viability was detected by methyl thiazolyl tetrazolium (MTT) assay. After 24 h treatment with juglone 20μmol·L-1,the cell apoptosis was detected by flow cytometry while the expressions of apopto?sis-related protein BCL-2,BAX and cleave-caspase-3,PI3K/AKt pathway-related protein PTEN,AKT and pAKT were detected by Western blotting. RESULTS After treatment with juglone for 24, 48 and 72 h,the growth of SiHa cells was significantly inhibited. Compared with cell control group,cells in juglone treated gruop were sparse,slipped off the wall,became round and the cell proliferation inhibitory rate was 43.3%,63.0%and 73.1%(P<0.05,P<0.01),respectively. Twenty-four hours post treatment, the early apoptosis rate of juglone treated gruop cells was increased by(6.47±1.79)%(P<0.01)compared with cell control group. Western blotting results showed that the expression of BCL-2 decreased by 53.0%while the expression of BAX and caspase-3 increased by 85.5%and 183.3%,respectively. The expression of PTEN was increased by 75.0% but the pAKt was decreased by 45.8%(P<0.01). CONCLUSION Juglone can upregulate the expression of PTEN, thus inhibiting PI3K/AKt pathway and promoting apoptosis of SiHa cells.
