Impact of different mouse blastocyst donors on the efficiency of germline chimera production from C57BL/6 embryonic stem cells
10.3969/j.issn.1005-4847.2015.04.005
- VernacularTitle:不同小鼠品系囊胚受体对 C57 BL／6胚胎干细胞种系嵌合效率的影响
- Author:
Shuya ZHOU
;
Qin ZUO
;
Susu LIU
;
Chenfei WANG
;
Baowen LI
;
Zhengming HE
;
Changfa FAN
- Publication Type:Journal Article
- Keywords:
C57BL/6 mouse embryonic stem cells;
BALB/c mouse blastocysts;
ICR mouse blastocysts;
B6 (Cg)-Tyrc-2J mouseblastocysts;
Germline transmission;
Germline chimera
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(4):353-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the gene targeting efficiency with C57BL/6 embryonic stem ( ES) cells.Meth-ods Three different genetically modified C57BL/6 ES cell lines, named TLX3, Ai3K and SL, were microinjected into ICR, B6( Cg)-Tyrc-2J and BALB/c mouse blastocysts, respectively.The efficiency was statistically evaluated according to three aspects:blastocyst collection, chimera production and germline transmission.Results None of the three ES cell lines was germline transmitted with B6(Cg)-Tyrc-2J mice as blastocyst donors, while it was achieved with both BALB/c and ICR mouse blastocysts.Compared in the aspect of blastocysts collection, ICR mouse was much better than BALB/c mouse (P<0.05), and the chimera production efficiency of ICR mouse was comparable to that of BALB/c mouse (P =0.115). As to the germline transmission efficiency, that of BALB/c mice is significantly higher than that of the ICR mice ( P<0.01).Conclusions The germline transmission efficiency of BALB/c mouse is highest among these three mouse strains. However, it has the disadvantages of blastocyst collection, developmental delay and zona pellucida fragility, compared with ICR mouse.Therefore, ICR mouse is also a good candidate as blastocyst donor for embryonic stem cell microinjection.
