Inhibition of autophgay enhances resveratrol-induced apoptosis of human chondrosarcoma cells
10.3969/j.issn.1000-4718.2015.08.009
- VernacularTitle:自噬抑制增强白藜芦醇对人软骨肉瘤细胞凋亡的诱导
- Author:
Kailiang ZHOU
;
Kai WU
;
Xiaolei ZHANG
;
Yongli WANG
;
Haidong JIN
;
Naifeng TIAN
;
Zhaojie CHEN
;
Huazi XU
- Publication Type:Journal Article
- Keywords:
Chondrosarcoma;
Resveratrol;
Autophagy;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2015;(8):1401-1406
- CountryChina
- Language:Chinese
-
Abstract:
AIM:Toinvestigatewhetherautophagyisup-regulatedwhenresveratrol(Res)inducesapoptosis in chondrosarcoma , and to study the effects of autophagy inhibitor combined with Res on chondrosarcoma .METHODS:SW1353 cells were divided into 4 groups: control group, Res group, 3-methyladenine (3MA) group, and Res +3MA group.Electron microscopy was used to observe the autophagyosomes in control group and Res group .At the same time, the viability of the cells in the 4 groups was detected by CCK-8 assay.TUNEL staining and Western blotting (for determi-ning the levels of cleaved caspase-3, Bax and Bcl-2) were used to reflect levels of apoptosis in all groups .The expression of autophagy-related proteins Beclin 1, LC3-Ⅱ and p62 was detected by Western blotting .RESULTS: Exposure of the cells to Res resulted in a decrease in cell viability and an increase in the level of apoptosis ( P<0.05 ) .Compared with control group, the level of apoptosis was increased but the autophagy was decreased (P <0.05).Compared with Res group, the cell viability and the level of autophagy were decreased and the level of apoptosis was increased ( P<0.05 ) . CONCLUSION:Resveratrol induces apoptosis and autophagy , and inhibition of autophgay enhances resveratrol-induced apoptosis in chondrosarcoma .
