Effect of PAK1 and LEF1 on esophagus cancer cell proliferation
10.3969/j.issn.1000-4718.2015.08.014
- VernacularTitle:PAK1和 LEF1对食道癌细胞增殖的影响
- Author:
Yong GU
;
Siyuan SHENG
;
Jun MA
;
Jianyong ZOU
;
Weijun OU
;
Chuangang LU
;
Rongfu WANG
;
Hai HONG
- Publication Type:Journal Article
- Keywords:
Esophaguscancer;
P21-activatedkinase;
lymphoidenhancer-bindingfactor1
- From:
Chinese Journal of Pathophysiology
2015;(8):1427-1431
- CountryChina
- Language:Chinese
-
Abstract:
AIM:ToinvestigatetheeffectofP21-activatedkinase1(PAK1)andlymphoidenhancer-binding factor 1(LEF1) on the proliferation of esophagus cancer cells .METHODS:Real-time PCR was applied to detect the mR-NA expression of PAK1 and LEF1 in the esophagus cancer tissues .MTT assay were used to measure the proliferation of hu-man esophagus cancer cell line KYSE transfected with PAK 1 and LEF1.RESULTS: The mRNA expression of PAK1 in the esophagus cancer tissues was lower than that in control group (P<0.05).The mRNA expression of LEF1 and tran-scription factor 4 (TCF4) in the esophagus cancer tissues was higher than that in control group (P<0.05).The prolifera-tion of KYSE cells with over-expression of PAK1 and LEF1 was higher than that in control group .No significant change of apoptosis between the KYSE cells with over-expression of PAK1 and LEF1 and control group was observed .CONCLU-SION:The expression of PAK1 decreases and the expression of LEF 1 increases in esophagus cancer tissues .LEF1 domi-nantly regulates the proliferation of esophagus cancer cells .
