Effects of siRNA-mediated nestin silencing on invasion and migration of human esophageal cancer ECA109 cells
10.3969/j.issn.1000-4718.2015.08.015
- VernacularTitle:siRNA 介导的 nestin 基因沉默对人食管癌ECA109细胞侵袭和迁移的影响及机制
- Author:
Huachuan WANG
;
Jianhu WEN
- Publication Type:Journal Article
- Keywords:
Nestin;
Neoplasminvasion;
Cellmigration;
Esophageal
- From:
Chinese Journal of Pathophysiology
2015;(8):1432-1436
- CountryChina
- Language:Chinese
-
Abstract:
AIM:ToinvestigatetheeffectofsmallinterferenceRNA(siRNA)-mediatedsilencingofnestin gene on the invasion and migration of human esophageal cancer ECA 109 cells and the possible mechanism .METHODS:The esophageal cell line ECA 109 was transfected with siRNA targeting nestin and the cell invasion and migration abilities were observed.The expression of nestin, MMP2, MMP9, VEGF, and total and nuclear β-catenin proteins in the transfec-ted cells were determined by real-time PCR and Western blot .RESULTS:Compared with control group , the expression of nestin at mRNA and protein levels was significantly down-regulated in the ECA109 cells transfected with nestin-siRNA, so was the expression of MMP2, MMP9, VEGF, and total and nuclear β-catenin proteins.The levels of invasion and migra-tion capacities of ECA 109 cells transfected with nestin-siRNA were lower than those in the cells transfected with control-siRNA.CONCLUSION:Knockdown of nestin expression significantly inhibits the invasion and migration of the esophage-al cancer cells , which may act via suppressing β-catenin translocation to the nucleus and influencing the expression of MMP2, MMP9 and VEGF.
