Determination of the activity of cytochrome C by the chromogenic substrate method
- VernacularTitle:生色底物法测定细胞色素C活力初探
- Author:
Lisha LIU
;
Yue WANG
;
Jing LI
;
Feifei LI
;
Huihong FAN
- Publication Type:Journal Article
- Keywords:
cytochrome C;
chromogenic substrate method;
activity;
substrate;
TMB
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(7):138-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the chromogenic substrate method to determine the activity of cytochrome C.Methods Used TMB as the chromogenic substrate, reacted at 37 ℃ for 15 min, generated the yellow products, and detected the absorbance at 450 nm.The experimental design method is the 4 ×4 parallel line quantitative analysis.ResuIts The activities of cytochrome C injection samples have been determined.The linear regression equation was Y=0.9875 X -1.0221,R2 =0.9996.The accuracy and repeatability were 1.1 % and 3.6 %.ConcIusion The chromogenic substrate method was simple operation, sensitive and can be used to determine the activity of cytochrome C.
