Progress of ion-pairing reverse liquid chromatography for analysis of oligonucleotides
- VernacularTitle:反相离子对色谱法对寡核苷酸药物分析的进展
- Author:
Hongmiao YANG
;
Huihong FAN
- Publication Type:Journal Article
- Keywords:
ion-pairing agents;
HPLC;
therapeutic oligonucleotides;
review
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(7):161-164
- CountryChina
- Language:Chinese
-
Abstract:
Therapeutic oligonucleotides have been one of the hottest areas in R&D of drugs in recent years.The research of analysis method has been widely carried out.Ion-pairing reverse liquid chromatography is the most commonly used method for good resolution and easy docking with MS.A review is presented here on the progress of oligonucleotides analysis with ion-pairing reverse liquid chromatography.The column, mobile phase, MS detection, mechanism of analysis will be discussed.
