Aspergillus fumigatus conidia inhibit early apoptosis of alveolar epithelial cells
10.3760/cma.j.issn.1000-6680.2015.07.011
- VernacularTitle:烟曲霉孢子对人肺泡上皮细胞早期凋亡的影响
- Author:
He SUN
;
Ting WU
;
Xiaoyong XU
;
Xin SU
;
Yi SHI
- Publication Type:Journal Article
- Keywords:
Aspergillus fumigatus;
Spores,fungal;
Tumor necrosis factor-alpha;
Staurosporine;
Epithelial cells;
Apoptosis
- From:
Chinese Journal of Infectious Diseases
2015;(7):420-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the impact of Aspergillus fumigatus conidia on the early apoptosis of alveolar epithelial cells.Methods A549 cells was first stimulated with tumor necrosis factor-α(TNF-α)or staurosporine (STS)to induce cell apoptosis,and then co-cultured with Aspergillus fumigatus conidia of three different concentrations (5 × 10 5 ,5 × 10 6 ,5 × 10 7 )simultaneously.The changes of the apoptosis rate of A549 cells were detected by flow cytometry.Results The early apoptosis of A549 cells was increased markedly after stimulated with TNF-α ([14.94 ± 0.82 ]% vs [3.26 ± 0.74]%,χ2 =9.18,P <0.05)and STS ([18.10±1 .00]% vs [3.26±0.74]%,χ2 =12.32,P <0.05). When Aspergillus fumigatus conidia of different concentrations were added,the apoptosis rate was decreased (χ2 =31 .23,P <0.05 ).The early apoptosis of A549 cells was negatively correlated with the concentration of Aspergillus fumigatus conidia (rTNF = - 0.92,r STS = - 0.94,both P < 0.05 ). Conclusion Aspergillus fumigatus conidia inhibits the early apoptosis of A549 cells induced by TNF-αor STS and the inhibit effect increases with the concentration of Aspergillus fumigatus conidia.
