Cloning and sequence analysis of methyltransferase gene in four species of Huperziaceae
- VernacularTitle:四种石杉植物甲基转移酶基因的克隆与序列分析
- Author:
Juncheng ZHANG
;
Zuoyi CHEN
;
Yuhong SONG
- Publication Type:Journal Article
- Keywords:
methyltransferase;
Huperziaceae;
gene cloning;
sequence analysis
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(7):13-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone and analyze the coding region of methyltransferase gene from four species of Huperziaceae, the species included Huperzia serrata var.longipetiolata, Phlegariurus minchegensis, Phlegariurus austrosinicus and Phlegariurus petiolatus.Methods The methyltransferase coding region sequences were cloned by RT-PCR with the template of total RNA extracted from four Huperziaceae.Then the sequences were analyzed by means of BLAST and MEGA 5.0 software.ResuIts The coding region sequences of the four species of Huperziaceae were highly similar to the methyltransferase in the database.ConcIusion The methyltransferase genes of the four species of Huperziaceae were cloned in this study.The result will provide a foundation for further elucidate the mechanism of Huperzine A biosynthesis and metabolic pathways in Huperziaceae plants.
