The changes and effects of Apelin/APJ system in LPS-induced injury of rat pulmonary microvascular endothelial cells
10.3969/j.issn.1001-1978.2015.08.025
- VernacularTitle:Apelin/APJ系统在脂多糖诱导大鼠肺微血管内皮细胞损伤中的变化及其作用研究
- Author:
Huanlong LIU
;
Zhongning ZHU
;
Ping JIANG
;
Yu HAN
;
Suwen SU
;
Xueyan CHEN
- Publication Type:Journal Article
- Keywords:
Apelin;
APJ;
lipopolysaccharide(LPS);
pulmonary microvascular endothelial cells(PMVECs);
proliferation;
Akt;
proliferating cell nuclear antigen(PCNA)
- From:
Chinese Pharmacological Bulletin
2015;(8):1152-1158
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the changes of Apelin/APJ system in LPS-induced injury of rat pulmonary mi-crovascular endothelial cells( PMVECs) , and the effect and mechanism of Apelin. Methods PMVECs were cultured with the explant technique, and the identifica-tion of rat PMVECs was carried out by immunocyto-chemical staining of factorⅧrelated antigen. MTT as-say was used to evaluate the viability of PMVECs. The mRNA expression of Apelin and APJ was detected by RT-PCR. The protein expression of PCNA and the phosphorylation of Akt was analyzed by Western blot. Results The mRNA expression of Apelin and APJ showed a compensatory increase after LPS treatment for a short period of time ( P<0. 01 ) , but with the exten-sion of time, which was significantly inhibited, even lower than the control group ( P<0. 05 or P<0. 01 ) , suggesting that Apelin/ APJ system might be involved in LPS-induced PMVECs injury. MTT results showed that 10 -6 ~10 -9 mol · L-1 Apelin obviously promoted the proliferation of rat PMVECs ( P <0. 05 or P <0. 01 ) , and with certain concentration and time de-pendence. Moreover, Apelin also improved the LPS-induced PMVECs injury in different degrees ( P<0. 05 or P < 0. 01 ) . In addition, Western blot analysis showed that Apelin significantly reversed the decrease of the protein expression of PCNA and the Akt phos-phorylation level induced by LPS ( P <0. 05 or P <0. 01 ) . Conclusions The Apelin/APJ system is in-volved in LPS-induced PMVECs injury. Apelin plays an important role in protecting the pulmonary microvas-cular endothelial function and reversing the LPS-in-duced PMVECs injury, which might be related to the activation of Akt phosphorylation pathway.
