DbcAMP inhibitted the proliferation of FTC-133 cell
10.3969/j.issn.1671-8348.2015.24.005
- VernacularTitle:双丁酰环腺苷酸抑制甲状腺癌细胞株 FTC-133增殖
- Author:
Yulin WANG
;
Hui WANG
;
Ying BA
- Publication Type:Journal Article
- Keywords:
thyroid neoplasms;
cell proliferation;
cyclic AMP;
dbcAMP;
Raf1
- From:
Chongqing Medicine
2015;(24):3325-3327
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of dualdi butyryl cyclic AMP (dbcAMP)on the proliferation of FTC-133 cell line. Methods FTC-133 cells were normally cultured and divided into control group,dbcAMP treatment group (0.5,1.0,2.0 mmol/L). After FTC-133 cells were treated with dbcAMP (0.5,1.0,2.0 mmol/L)for 24 h or 48 h,the growth activity and growth curve was detected by MTT.Changes of the cell cycle were detected by flow cytometry.The mRNA and protein expression of Raf1 were measured by RT-qPCR and Western blotting.Results Compared with control group,the growth activity of FTC-133 cells was re-duced by different levels of dbcAMP in a dose-time dependence manner.The number of FTC-133 cells was decreased in the S phase and increased in the G2/M phase.The mRNA and protein expression of Raf1 of treatment group were both reduced compared with control group.Conclusion dbcAMP significantly reduced FTC-133 cells proliferation and promoted apoptosis,and which might be involoved by ERK MAPK signalling.
