Apoptotic effect of HBO exposure on cerebral vascular endothelial cells in rabbits
10.3969/j.issn.1671-8348.2015.24.006
- VernacularTitle:高压氧对兔颈内动脉和基底动脉内皮细胞凋亡影响的实验研究?
- Author:
Zhenzhen FAN
;
Zhiyan LIU
;
Hongbin CAI
;
Cuijie ZHANG
;
Xudong ZHANG
;
Lanfang ZHANG
;
Cheng LIANG
;
Zhaoming GE
- Publication Type:Journal Article
- Keywords:
hyperbaric Oxygen;
internal carotid artery;
basal artery;
cell apoptosis;
Caspase-3;
Bcl-2;
Real-time F.Q.PCR
- From:
Chongqing Medicine
2015;(24):3328-3330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hyperbaric Oxygen (HBO)exposure on Caspase-3 and Bcl-2 mRNA ex-pressions in both internal carotid artery (ICA)and basal artery (BA)in rabbits.Methods Twenty-four healthy adult New Zealand rabbits were randomly divided into 2 groups:HBO group and the control group,with each group consisted of 12 animals.The rab-bits in the HBO group were exposed to HBO at 2.2 ATA for 60 minutes each day for 3 successive days.The rabbits in the control group were normally fed without any treatment.Real-time PCR was used to detect Caspase-3 and Bcl-2 mRNA expressions in both ICA and BA in 2 groups.Results HBO significantly decreased the Caspase-3 mRNA expression [(0.038 ±0.006 )vs .(1.000 ± 0.225)]and increased the Bcl-2 mRNA expression [(1.877 ±0.1 69)vs .(1.000 ±0.364)].In ICA,HBO similarly decreased the Caspase-3 mRNA expression [(0.41 9±0.091)vs .(1.000 ±0.1 75)]and increased the Bcl-2 mRNA expression [(1.269 ±0.270) vs .(1.000±0.1 1 7)]in BA.All the differences mentioned above were of statistical significance (P <0.01).Conclusion HBO ex-erts an inhibition effect on apoptosis in cerebral vascular endothelial cells.The mechanism may be related to inhibiting the expres-sion of Caspase-3 mRNA and promoting the expression of Bcl-2 mRNA.
