The immunoregulative action of polysacchrides of Spirulina platensis in peripheral blood mononuclear cells in patients with chronic hepatitis B virus infection
10.3969/j.issn.1001-1978.2015.08.019
- VernacularTitle:螺旋藻多糖对CHB患者PBMC免疫功能的影响
- Author:
Xiaohua LYU
;
Wenqing CHEN
;
Shiying LUO
;
Tie WU
;
Shijing LYU
- Publication Type:Journal Article
- Keywords:
polysacchrides of Spirulina platensis;
chronic hepatitis B;
peripheral blood mononuclear cells;
interferon;
interleukin-2;
interleukin-4
- From:
Chinese Pharmacological Bulletin
2015;(8):1121-1125
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the immunoregulative action of polysacchrides of Spirulina platensis ( PSP ) on peripheral blood mononuclear cells ( PBMCs) in pa-tients with chronic hepatitis B virus infection. Meth-ods Proliferation and cycle of PBMCs from patients with chronic hepatitis B virus infection were measured by MTT colorimetry and flow cytometry respectively. The concentration of IFN-γ, IL-2 , IL-4 in supernatants of PBMCs were examined by ELISA. The mRNA ex-pression of IFN-γin PBMCs from patients with chronic hepatitis B virus infection was detected by RT-PCR. Results PSP could induce proliferation of PBMCs in patients with chronic hepatitis B virus infection. In ad-dition, PSP could increase the protein expression of IFN-γ and IL-2 in supernatants of PBMCs in patients with chronic hepatitis B virus infection, and PSP could also enhance the mRNA expression of IFN-γof PBMCs in patients with chronic hepatitis B virus infection. However, PSP inhibited the protein expression of IL-4 of PBMCs in patients with chronic hepatitis B virus in-fection. Conclusion PSP can promote the immune function and plays an important immunoregulation function on patients with chronic hepatitis B virus in-fection.
