Effects of Epigallocatechin gallate on IL-1βinduced MIN6 cells apoptosis
10.3969/j.issn.1671-8348.2015.23.005
- VernacularTitle:表没食子儿茶素没食子酸酯对 IL-1β诱导 MIN6细胞凋亡的保护作用?
- Author:
Hua LIU
;
Diyong CAO
;
Shangjun YANG
;
Hong LIU
;
Mei YANG
;
Xin ZHANG
;
Keli WEN
;
Qian ZHENG
- Publication Type:Journal Article
- Keywords:
catechin;
gallic acid;
adenoma,islet vell;
mitochondrial membrane potential;
adenosine triphosphate;
reactive oxy-gen species
- From:
Chongqing Medicine
2015;(23):3183-3186
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Epigallocatechin gallate(EGCG)on IL-1βinduced MIN6 cells apoptosis. M.Methods The experiment group was divided into control group,IL-1β group,IL-1β+ EGCG low concentration group and IL-1β+EGCG high concentration group.Cell activity was detected by CCK8.Insulin secretion was detected by ELISA.cell apoptosis was detected by flow cytometry.The mitochondrial membrane potential was detected by flow cytometry.ATP content and cell ac-tivity of ROS were detected by colorimetry and chemiluminescence method.Results Compared with normal group,IL-1β group showed much lower cell activity,insulin secretion,cell mitochondrial membrane potential and ATP content,and at the same time IL-1βgroup had significantly higher cell apoptosis and ROS activities.After given EGCG,both low concentration group and high con-centration group had higher cell activity,insulin secretion,cell mitochondrial membrane potential and ATP content,at the same time lower cell apoptosis and ROS activities was showed.And the IL-1β+EGCG high concentration group worked more powerful.Con-clusion EGCG has protective effects on IL-1βinduced MIN6 cells apoptosis.Its mechanism may be related to increasing the content of the ATP and mitochondrial membrane potential and protecting mitochondrial function as well reducing the activity of ROS.
