Establishment of Tp0259-PCR for diagnosing infection of treponema pallidum
10.3969/j.issn.1673-4130.2015.15.045
- VernacularTitle:梅毒感染诊断中Tp0259-PCR方法的建立
- Author:
Changshun YANG
;
Sanhu WANG
;
Xiuping ZHOU
- Publication Type:Journal Article
- Keywords:
treponema pallidum;
polymerase chain reaction;
gene
- From:
International Journal of Laboratory Medicine
2015;(15):2232-2233
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for detection Tp0259 gene by PCR .To provide basis of diagnosis infection of trepo‐nema pallidum by detection Tp0259 gene .Methods Gene sequences of Tp0259 was obtained from Genebank and analysis by bioin‐formatics .Specific primers were designed .The whole genome was extracted from testis specimens of the New Zealand rabbits infec‐ted by treponema pallidum and control groups .The gene of Tp0259 was amplified by PCR under the optimization reaction condi‐tions which was selected by many tests ,using the new construction of amplification method to detect the common pathogenic micro‐organism in urinary and reproductive tract infection .Results Specific bands of 646 bp was appeared only in New Zealand rabbits which was infected by treponema pallidum ,while none was in the control group and other pathogenic microorganisms .Conclusion Detection the Tp0259 gene could be used to diagnose infection of treponema pallidum .
