In vitro fabrication of a tissue engineering nucleus pulposus with nucleus pulposus acellular matrix scaffold and bone marrow mesenchymal stem cells
10.3969/j.issn.1671-8348.2015.23.003
- VernacularTitle:髓核去细胞基质复合骨髓间充质干细胞体外构建组织工程髓核的研究?
- Author:
Wei TAN
;
Hai LV
;
Wei CAO
;
Peng ZHANG
;
Liuzhu YANG
;
Chusong ZHOU
- Publication Type:Journal Article
- Keywords:
tissue engineering;
scaffold;
nucleus pulposus;
acellular matrix;
bone marrow mesenchymal stem cells
- From:
Chongqing Medicine
2015;(23):3176-3179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct tissue engineering nucleus pulposus by culture of rabbit bone marrow mesenchymal stem cells (rBMSCs)-nucleus pulposus acellular matrix scaffold (NPAMS)complexes (rBMSCs-NPAMS).Methods Several NPAMS were prepared,and rBMSCs was seeded into NPAMS.The scaffolds and complex were detected by general observation,HE stai-ning,immunohistochemical,qRT-PCR,scanning electron microscopy.Results The scanning electron microscopy showed the seed cell in nucleus pulposus ECM-derived scaffold could adhesion and growth.The cell attachment and proliferation were observed by HE staining.Immunohistochemical examination with typeⅡ collagen showed positive results.qRT-PCR revealed the time-depend-ent of the mRNA expression of collagen Ⅱ,and which was smaller than positive control(P<0.01).The relative expression of Ag-grecan mRNA grew in a time dependent fashion,which was still lower than positive control(P<0.01).Scaffolds and normal nucle-us pulposus had no statistical significance of the compression load under the same displacement of the compression(P>0.05).Con-clusion Natural nucleus pulposus acellular matrix scaffold composite allogeneic bone marrow mesenchymal stem cells can be suc-cessfully built into tissue engineering nucleus pulposus.
