The diagnostic value of enzyme-linked immunospot assay in AIDS patients with Mycobacterium tuberculosis infection
10.3969/j.issn.1673-4130.2015.20.037
- VernacularTitle:酶联免疫斑点法对 AIDS 患者感染结核分枝杆菌的诊断价值
- Author:
Peng JIANG
- Publication Type:Journal Article
- Keywords:
enzyme-linked immunospot;
human immunodeficiency virus;
acquired immunodeficiency syndrome;
tuberculo-sis
- From:
International Journal of Laboratory Medicine
2015;(20):3009-3010
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the value of enzyme‐linked immunospot(ELISPOT ) assay in the diagnosis of acquired immu‐nodeficiency syndrome(AIDS) patients with Mycobacterium tuberculosis infection .Methods 42 hospitalized AIDS patients with Mycobacterium tuberculosis infection and 60 non‐tuberculosis hospitalized patients with diseases of respiratory system in the same period were recruited as observation group and control group ,respectively .ELISPOT assay ,tuberculosis antibody(TBAb) test and tuberculin skin test(TST) were performed for all the patients ,respectively .The sensitivity ,specificity and accuracy of the 3 meth‐ods were comparatively analyzed .Results The sensitivity ,specificity and accuracy of ELISPOT were 92 .9% ,93 .3% and 93 .1% . These of TBAb were 54 .8% ,71 .7% and 64 .7% ,and of TST were 47 .6% ,53 .3% and 51 .0% ,respectively .There were significant differences among three methods in sensitivity ,specificity and accuracy(P<0 .05) .The sensitivity ,specificity and accuracy of ELIS‐POT were the highest in the 3 methods(P<0 .05) .There were no significant differences between TBAb test and TST in sensitivi‐ty ,specificity and accuracy(P>0 .05) .Conclusion ELISPOT assay has relatively high diagnostic value in AIDS patients with My‐cobacterium tuberculosis infection .
