Role of activin A and NGF in co-stimulating neurite outgrowth of dorsal root ganglia ofembryonic chicken
10.3969/j.issn.1000-484X.2015.09.012
- VernacularTitle:激活素 A与NGF协同刺激鸡胚背根神经节神经突起生长
- Author:
Lin FANG
;
Yinan WANG
;
Jingyan GE
;
Haiyan LIU
;
Chenguang LI
;
Jiru LI
;
Zhonghui LIU
- Publication Type:Journal Article
- Keywords:
Activin A;
Nerve growth factor ( NGF);
Neurite outgrowth;
CGRP
- From:
Chinese Journal of Immunology
2015;(9):1206-1209
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of activin A and nerve growth factor ( NGF) NGF on stimulating neurite outgrowth of dorsal root ganglia(DRG)of the embryonic chicken.Methods:In this study,we observed that activin A and NGF together induced neurite outgrowth of DRG and kept survival of DRG neurons by the primary cultured DRGs from embryonic day 8 ( E8 ) chicken.calcitonin gene-related peptide(CGRP)CGRP mRNA expressions were analyzed by RT-PCR.Results: The DRG treated with activin A +NGF had obvious neurite outgrowth ,compared with only NGF group on day 3,and the number of living DRG neurons also increased.Activin A +NGF up-regulated the mRNA expressions of CGRP in DRG.Conclusion:The Data demonstrated that activin A with NGF can synergistically stimulate DRG neurite outgrowth and maintain the DRG neurons survival , suggesting that it is more effective that NGF and activin A together treat the associated disease of nerve system.
