Impact of miR-873 on human prostate cancer PC3 cells invasion

VernacularTitle:miR－873靶向生存蛋白基因对人前列腺癌PC3细胞侵袭性的影响
Author: Tan LI ; Chenguang LI ; Yan LIU ; Bingxun LI ; Xianxu YANG
Publication Type:Journal Article
Keywords: miRNA; survivin; prostate cancer cells PC3
From: Military Medical Sciences 2015;(8):610-613
CountryChina
Language:Chinese
Abstract: Objective To identify the role of miR-873, which may regulate the expression of survivin,in human pros-tate cancer PC3 cells invasion.Methods PC3 cells were cultured in vitro, and changes of cellular morphology were ob-served by phase contrast microscope.miR-873,which might regulate the expression of survivin,was predicted by bioinforma-tics and identified using dual luciferase report system.Expressions of miR-873 and survivin were determined using real-time quantitative PCR( qRT-PCR) and Western blotting after transfection of miR-873 mimics.The invasion of PC3 cells was de-tected in vitro by Transwell chamber.Results The expression of survivin was positive by immunofluorescence cytochemis-try.Using dual luciferase reporter system, miR-873 could inhibit the expression of survivin by binding to its mRNA 3′UTR.Results of qRT-PCR and Western blotting showed that overexpression of miR-873 down-regulated the expression of survivin.The invasion of PC3 cells was suppressed by over-expression of miR-873.Conclusion MiR-873 may negatively regulate the expression of survivin in human prostate cancer PC3 cells and inhibit cell invasion.