Pharmacokinetic and Intestinal Absorption Study on Rats of Emodin in Colon-Targeted Huchang Qingdu Pellets
10.3969/j.issn.1005-5304.2015.09.022
- VernacularTitle:结肠靶向护肠清毒微丸中大黄素在大鼠体内肠吸收及药物动力学研究
- Author:
Ping SUN
;
Chuanjiang MA
;
Leyang LIU
- Publication Type:Journal Article
- Keywords:
Huchang Qingdu Pellets;
emodin;
colon targeting;
pharmacokinetics
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(9):79-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for concentration determination of emodin in plasma and colon tissues after gavage withHuchang Qingdu Pellets;To explore pharmacokinetic properties and colon tissue distribution ofHuchang Qingdu Pellets in rats.Methods Wistar rats were randomly divided into Pellets group and ordinary capsule group. Blood was taken after gavage withHuchang QingduPellets or capsule content in given time points (1, 2, 3, 4, 5, 6, 8, 12, 24 h) from rat venous sinus and analyzed. Rats were put to death, and gastric and colon tissues were isolated and extracted for analysis. Chromatographic conditions:Phenomenex C18 column (4.6 mm × 150 mm, 10μm), the mobile phase of methanol-0.1% acetic acid solution (80∶20), flow rate of 1 mL/min, the detection wavelength of 254 nm.Results Emodin was in good linear range of 0.102-20.40μg/mL. The regression equations were:plasma solutionA=76 825C + 3567.8,r=0.999 4 (n=6);intestinal tissue solutionA=75 931C + 3384.2,r=0.999 0 (n=6). Day-to-day precision and within-day precision were less than 4%. The recovery rate was more than 90%. Pharmacokinetic parameters of T1/2 and Tmax of emodin in rat blood and intestinal tissues increased compared with the ordinary capsule group;Cmax and AUC0-24 were obviously improved compared with the ordinary capsule group.ConclusionHuchang Qingdu Pellets do have controlled release and colon targeting effect.
