Enhancing effect of astragalus polysaccharide on the immune function in cytoxan-induced immunosuppressed mice
10.3969/j.issn.1005-4847.2015.04.011
- VernacularTitle:黄芪多糖干预环磷酰胺所致免疫抑制小鼠的免疫功能
- Author:
Ruiqi ZHANG
;
Zhengli CHEN
;
Qihui LUO
- Publication Type:Journal Article
- Keywords:
Astragalus polysaccharide;
Mice;
Cytoxan;
Immune function
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(4):389-394
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the regulatory effect of astragalus polysaccharide ( APS) on immune function in immunosuppressed mice, and to establish a set of feasible indexes of immunological regulation and evaluation system of pol-ysaccharides.Methods Both Cytoxan ( CTX)-induced immunosuppressed mice and normal mice were treated with astrag-alus polysaccharide ( APS) .Spleen index, thymus index, phagocytosis rate and phagocytic index of peritoneal macrophages were assessed, and the spleen and thymus tissues were examined by histology.The percentage of CD3 +, CD4 +, and CD8 +cells in peripheral blood were determined by flow cytometry.Results APS significantly increased the thymus and spleen indexes in both immunosuppressed mice and normal mice, promoted the histological development of spleen and thy-mus in normal mice, enhanced the recovery of histological structures in the spleen and thymus in the CTX-induced immuno-suppressed mice.APS also enhanced the phagocytosis rate and phagocytic index of peritoneal macrophages in both immuno-suppressed mice and normal mice.In addition, the percentage of CD3 +and CD4 +cells,the ratio of CD4 +/CD8 +in pe-ripheral blood of both CTX-induced immunosuppressed mice and normal mice were increased while CD8 +cells were de-creased.Conclusions The results of this study suggest that astragalus polysaccharide can improve the non-specific immu-nity and cellular immunity in mice, and indicate that we established comprehensive evaluation indexes of immunoregulatory effects of polysaccharides.
