Simultaneous Determination of 6 Aconitum Alkaloids in CompoundTengwu Ointment by HPLC
10.3969/j.issn.1005-5304.2015.09.024
- VernacularTitle:HPLC同时测定复方藤乌软膏中6种乌头类生物碱含量
- Author:
Meiyan LI
;
Qingxue HE
;
Aiwu WANG
- Publication Type:Journal Article
- Keywords:
compoundTengwu Ointment;
aconitine;
mesaconition;
hypaconitine;
benzoylaconine;
benzoylmesaconine;
benzoylhypaconine;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(9):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the simultaneous determination of the 6 aconitum alkaloids in compoundTengwu Ointment.Methods Chromatographic column was Aglient XDB-C18 column (4.6 mm × 250 mm, 5μm), with the mobile phase A of acetonitrile- tetrahydrofuran (25∶8), phase B was 0.1 mol/L ammonium acetate solution (0.5 mL acetic acid added to the 1000 mL) with gradient elution;the flow rate was 1 mL/min;the detection wavelength was set at 235 nm;the column temperature was 25℃.Results Aconitine was in the good linear range of 0.072-0.648μg (r=0.999 5), with the average recovery of 99.29%, RSD=1.25%. Mesaconitine was in the good linear range of 0.062-0.648μg (r=0.999 5), with the average recovery of 99.12%, RSD=0.85%. Hypaconitine was in the good linear range of 0.064-0.384μg (r=0.999 8), with the average recovery of 99.57%, RSD=1.07%. Benzoylaconine was in the good linear range of 0.056-0.672μg (r=0.999 2), with the average recovery of 98.11%, RSD=0.61%. Benzoylmesaconine was in the good linear range of 0.055-0.993μg (r=0.999 9), with the average recovery of 99.27%, RSD=1.10%. Benzoylhypaconine was in the good linear range of 0.078-0.702μg (r=0.999 8), with the average recovery of 99.08%, RSD=1.38%.Conclusion This method is simple, sensitive, repeatable and accurate, which can be used for determination of aconitum alkaloids in compoundTengwu Ointment.
