Effect ofGuizhi-Fulingcapsule on expression of AngⅡ, eNOS in rats with UUO-induced tubulointerstitial fibrosis
10.3760/cma.j.issn.1673-4246.2015.08.014
- VernacularTitle:桂枝茯苓胶囊预防肾小管间质纤维化及对大鼠肾组织血管紧张素Ⅱ、内皮型一氧化氮合酶表达的影响
- Author:
Shujun LI
;
Jianjun LIU
;
Yan ZHANG
;
Xiyan JIA
;
Zuozhen CHAI
;
Dasheng YANG
- Publication Type:Journal Article
- Keywords:
Kidney tubules;
Fibrosis;
Angiotensin II;
Nitric oxide synthase;
Guizhi-Fuling capsule;
Rats
- From:
International Journal of Traditional Chinese Medicine
2015;(8):724-727
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To observe the effect ofGuizhi-Fuling capsule on the expression of AngⅡand eNOS.Methods 72 male Sprague-Dawley(SD) rats, aged three months, were randomly divided into three groups: a sham-operated group(n=24),a model group (n=24) and a treatment group (n=24). Renal tubulointerstitial fibrosis model was established via unilateral ureteral obstruction(UUO). Rats in the treatment group were treated withGuizhi-Fulingcapsule (125mg/kg),and the same volume of normal saline were given to the rats in the sham -operated group and the model group at the same time. Rats were put to death at day 7, 14, 21 Immunohisto-chemistry staining was performed to investigate both the expression of AngⅡ, eNOS in TIF. Results 1 Renal interstitium injury was seen in the model group and the treatment group rats at day 7. The degree of renal interstitium fibrosis in rats from the treatment group was lighter than that of the model group at day 7, 14, 21 (P< 0.01).2 At day 7,14 and 21, the expression of AngⅡin rats from the model group and the treatment group were higher than that of the sham-operated group (P<0.01), but those of the treatment group was lower than those of the model group (P< 0.01). The expression of AngⅡ in rats from the model and the treatment groups were increasing,the highest in the 21th day (P<0.01).3 At day 7, 14 and 21, the expression of eNOS in rats from the model group and the treatment group were lower than that of the sham-operated group (P<0.01).ConclusionsGuizhi-Fuling capsule can alleviate the lesion of renal tissue in obstructive kidney and delay the development of TIF.
