Study on NO Inhibitory Activity of Total Lactones fromAndrographis paniculata
10.11842/wst.2015.05.027
- VernacularTitle:穿心莲总内酯的NO抑制活性研究*
- Author:
Fangfang XU
;
Zhiquan JIN
;
Wei SHI
;
Yimin MA
;
Jun ZHOU
;
Zhenzhong WANG
;
Yuan BI
;
Zhimin WANG
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
Andrographis paniculata;
total lactones;
andrographolide;
nitric oxide;
inhibitory rate
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(5):1061-1065
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the suppression of nitric oxide (NO) production in RAW264.7 cells by total lactones fraction from Andrographis paniculata. The inflammatory model in vitro was established by stimulating the RAW264.7 cells with lipopolysaccharide (LPS). The NO production and inhibitory rate were determined by Griess assay. Cytotoxicity was evaluated by MTT method. The results showed that total lactones fraction ofA. paniculata suppressed NO production in a concentration-dependent manner in the concentration range from 5 to 50μmol·L-1 and their IC50 values were 8.58μmol·L-1, 11.52μmol·L-1 and 8.94μmol·L-1, respectively. In this condition, major constituents were andrographolide (5-60μmol·L-1), dehydroandrographolide (5-100μmol·L-1) and neoandrographolide (5-100μmol·L-1) inhibited NO production in a dose-dependent manner with an IC50 values of 17.54μmol·L-1, 49.54μmol·L-1 and 41.80μmol·L-1, respectively. It was concluded that the NO inhibitory activity of total lactones fromA. paniculata was better than three active ingredients, which may be able to provide a theoretical foundation and scientific basis for the preparation and clinical application of total lactones fraction fromA. paniculata.