Expression and significance of changes of TLR4 and IL-6 in hippocampal CA1 area of rats after ischemia adaptation in distance
10.3969/j.issn.1000-484X.2015.08.009
- VernacularTitle:远程缺血后适应中大鼠海马CA1区TLR4和IL-6的表达变化及其意义
- Author:
Jiang ZHANG
;
Lisha CHANG
;
Dali WANG
;
Tao ZHOU
;
Huiying LIU
- Publication Type:Journal Article
- Keywords:
Remote ischemic postconditioning;
TLR4;
IL-6;
Expression
- From:
Chinese Journal of Immunology
2015;(8):1045-1048
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes of TLR4 and IL-6 expression in rats hippocampus CA1 region in remote ischemic postconditioning(RIP) and explore its significance.Methods: All the 72 male SD rats were divided into Sham group,Contrast group and RIP group randomly.Each group was divided into 4 time points:12h,24 h,48 h and 72 h group.There were 6 rats in each group.Use the cerebral ischemia-reperfusion model which was established with modified Longa method as the contrast group.The method of RIP was to Fasten rats Posterior limbs by a tourniquet for 30 min immediately,then relax them for 30 min, repeat 3 times.To observe the pathological variation of hippocampus CA1 region by HE dyeing;to test the expression of TLR4 and IL-6 by immunohistochemical staining.Results: Compared to contrast group, neuronal loss and swelling reduced significantly in RIP group.Compared to sham group, the TLR4 and IL-6 expression in contrast group and RIP group increased significantly ( P<0.05 ) .Compared to contrast group,the TLR4 and IL-6 expression in RIP group reduced significantly(P<0.05).Conclusion:RIP dose have protective effect on cerebral ischemia.The effect may be associated with the inhibition of TLR4 and IL-6 expression.
