Transformation of PPS induce M2 subtype to M1 macrophages
10.3969/j.issn.1000-484X.2015.08.010
- VernacularTitle:猪苓多糖诱导M2亚型巨噬细胞向M1巨噬细胞转化
- Author:
Zebo JIANG
;
Jin ZHAO
;
Siming LI
;
Jinping HU
;
Xing ZENG
- Publication Type:Journal Article
- Keywords:
PPS;
M2 subtype macrophages;
M1 macrophages;
Membrane surface molecules
- From:
Chinese Journal of Immunology
2015;(8):1049-1052
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the positive expression rate of M2 subtype of macrophage cell surface molecules and the inflammatory factors of PPS in IL-4-induced M2 macrophage.Methods:The experiment was divided into 5 groups:blank control group, Model group,PPS groups(50 μg/ml,100 μg/ml and 200 μg/ml).The expression of CD206 and CD23 was used as bio-maker to confirm IL-4 induced macrophages by treating RAW264.7 with 20ng/ml of IL-4.IL-4 induced RAW264.7 cells were treated with PPS of 50μg/ml,100μg/ml and 200μg/ml for 24 h.Then the expression of CD206,CD16/32 and CD40 were analyzed by flow cytometry, and the mRNA expression of IL-1β,TNF-α,IL-10 and iNOS were detect by qRT-PCR.Results: After treated with IL-4,the positive rate of CD206 of RAW264.7 were high.After treated with PPS ,the rate of CD16/32 and CD40 in IL-4 induced RAW264.7 cells were high ,the expression of CD206 decreased,and the mRNA level of IL-1βand TNF-αincreased.Conclusion:RAW264.7 cells can be polarlized to M2 subtype macrophage by using 20 ng/ml IL-4.PPS enhances the mRNA of IL-1β,TNF-αand the expression of CD40, CD16/32 in IL-4-induced RAW264.7 cells .These results indicate that PPS can induce the M2 subtype to become M1 macrophages, can improve immune function of macrophages.
