Inhibitory of ginsenoside on proliferation of human thyroid cancer cells and expression of C -myc and Bcl -2 protein in vitro
10.3760/cma.j.issn.1008-6706.2015.16.012
- VernacularTitle:人参皂苷抑制甲状腺癌 SW579细胞增殖和 C-myc、Bcl-2蛋白表达的研究
- Author:
Ruoming WANG
;
Zhen YANG
- Publication Type:Journal Article
- Keywords:
Cancer cell;
Thyroid neoplasm;
Gisenoside;
C -myc protein;
Bcl -2 protein
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(16):2437-2440
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore inhibitory effects of ginsenoside on the proliferation of human thyroid canc-er SW579 cells in vitro and expression of C -myc and Bcl -2 protein.Methods Human thyroid cancer SW579 cells were cultured according to conventional method.The study was divided into the control group and ginsenoside group (20,40,8ug/mL).Inhibitory role of ginsenoside on proliferation of SW579 cells was detected by MTT assay.Western-blot method was used to determine expression levels of C -myc and Bcl -2 protein in different group.Results The inhibition rates of 20,40,80μg/mL ginsenoside to SW579 thyroid carcinoma cell were 22.35%,51.76% and 68.24% respectively.Which meaned ginsenoside had obvious inhibitory effect compared with the control group(P <0.01),and inhibition increased with the increase of solubility(P <0.01).C -myc and Bcl -2 protein were reduced progressively in place with the increase of ginsenoside concentration by Western -Blot analysis (P <0.01 ).Conclusion Gisenoside may play the inhibitory role on proliferation of human thyroid cancer SW579 cells in vitro, and its mechanism may be related with down -regulation of C -myc and Bcl -2 protein.
