Application of high hydrostatic pressure in development of melanoma vaccine
10.3969/j.issn.1000-484X.2015.08.017
- VernacularTitle:探索静水高压在研制黑色素瘤疫苗方面的应用
- Author:
Kai LIU
;
Zeqiang LIU
;
Minli ZHUANG
;
Yuehai PAN
;
Zhanchuan MA
;
Bin LIU
- Publication Type:Journal Article
- Keywords:
Fluorescence imaging;
High hydrostatic pressure;
Liquid nitrogen freezing and thawing;
Melanoma vaccine
- From:
Chinese Journal of Immunology
2015;(8):1077-1081
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the application of high hydrostatic pressure in the development of melanoma vaccine.Methods:The high hydrostatic pressure,liquid nitrogen freezing and thawing were used to break the murine B16 melanoma cells and then the cell suspension was mixed with Freund's adjuvant to prepare vaccine for immunizing the mice.Results:After immu-nization,the murine B16 melanoma cells were injected intravenously and subcutaneously.The immune results of the vaccines were evaluated,by comparing survival time of the mice, subcutaneous tumor volume, DTH experiments and the fluorescence imaging of tumors.Conclusion:Compared with the method of liquid nitrogen freeze-thaw broken,high hydrostatic pressure crushing cells has more advantages in the development of tumor vaccine.
