Study on expression of caspase-3 and free radical injury in hypoxic-ischemic brain damage of neonatal rats
10.3969/j.issn.1000-484X.2015.08.021
- VernacularTitle:新生大鼠缺氧缺血性脑损伤caspase-3表达与自由基损伤机制的研究
- Author:
Kunming YAN
;
Ying LIU
;
Rui ZHANG
;
Shijie GUO
- Publication Type:Journal Article
- Keywords:
Hypoxic-ischemic brain damage;
Caspase-3;
Free radical;
Neonatal rat
- From:
Chinese Journal of Immunology
2015;(8):1094-1097
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of caspase-3 and free radical injury in hypoxic-ischemic brain damage ( HIBD) of neonatal rats.Methods:All seven-day-old Wistar rats were randomly divided into HIBD group and sham operation group.Brains was obtained at time of 6 h,12 h,24 h,48 h,72 h,96 h after HIBD.Neuronal cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling ( TUNEL).The expression level of caspase-3 protein was detected by immunohisto-chemistry.The level of malondialdehyde ( MDA) and supemxide dismutase ( SOD) were measured by thiobarbitic acid colorimetry and xanthin oxidase,respectively.Results:The number of neuronal cell apoptosis and the expression of caspase-3 protein began to increase at 6 h and reached the peak at 48 h in HIBD group,they were both significantly higher than those in the sham operation group at each time point (P<0.05).The level of MDA began to increase at 6 h and reached the peak at 24 h in HIBD group,it was significantly higher than the sham operation group at each time point (P<0.05).The level of SOD began to decrease at 6 h and reached the lowest level at 24 h in HIBD group,it was significantly lower than the sham operation group at each time point ( P<0.05 ) .The number of neuronal cell apoptosis and the expression of caspase-3 protein were positively correlated with the level of MDA, but they were negatively correlated with the level of SOD.Conclusion: Free radical injury promotes the expression of caspase-3 and neuronal cell apoptosis in HIBD.
