Effect ofQiwei Granules on Renal Podocyte Injury in Diabetic Nephropathy Mice
10.11842/wst.2015.06.006
- VernacularTitle:芪卫颗粒干预糖尿病肾病小鼠足细胞损伤的作用研究＊
- Author:
Jingxin ZHOU
;
Wen SUN
;
Yan ZHANG
;
Lili WU
;
Lingling QIN
;
Xuan GUO
;
Guangyuan XU
;
Linyi LI
;
Ying DUAN
;
Xiangyu GUO
;
Xinli WU
;
Tonghua LIU
- Publication Type:Journal Article
- Keywords:
Qiwei granules;
diabetic nephropathy;
podocyte injury;
KK-Ay mice
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(6):1150-1156
- CountryChina
- Language:Chinese
-
Abstract:
This article was aimed to study the effect ofQiwei granules on the podocyte in KK-Ay mice kidney. The 28 8-week-old male KK-Ay mice were randomly divided into the model group, low-dosage, middle-dosage and high-dosageQiwei granule group. Eight C57BL/6J mice were used as the normal control. The general conditions, blood glucose and 24 h albuminuria were recorded in the experiment. After 10-week treatment, renal indexes including serum creatinine and urea nitrogen were measured. The kidneys of mice were collected and measured. The hematoxylin and eosin (HE), Masson’s Trichrome, and periodic acid-Schiff (PAS) were used on renal tissues of mice. The immunohistochemical staining for WT-1 was made. Software analysis was combined in the calculation of renal podocyte amount. Western blot was used in the detection of nephrin protein expressions in the kidney of mice. RT-PCR was used in the detection of nephrin mRNA expression. The results showed that compared with the model group, the body weight, blood glucose, 24 h albuminuria and the serum creatinine were obviously decreased after 10-week treatment ofQiwei granules. It can effectively improve the glomerular mesangial proliferation and preserve the podocyte number. Meanwhile, after the treatment ofQiwei granules, the nephrin protein expression and mRNA expression were obviously higher than the model group. It was concluded thatQiwei granules probably managed nephrin expression to improve the podocyte injury in the diabetic nephropathy of KK-Ay mice.
