Ameliorative Effect ofGuava Leaf Total Flavonoids Reversing Insulin Resistance in Pancreaticβ Cells
10.11842/wst.2015.06.008
- VernacularTitle:番石榴叶总黄酮对胰岛β细胞胰岛素抵抗影响的实验研究＊
- Author:
Xiaoguang YAN
;
Wen SUN
;
Tonghua LIU
;
Tunhai XU
;
Xuan GUO
;
Lili WU
;
Xiaohong MU
;
Xiangyu GUO
;
Ying DUAN
- Publication Type:Journal Article
- Keywords:
Guava leaf;
total flavones;
HIT-T15 pancreaticβ cell;
insulin resistance
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(6):1164-1168
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the effect ofGuava leaf total flavonoids on HIT-T15 pancreaticβcell insulin resistance. Effective part of FSL was prepared. The dosing time, concentration and high glucose concentration of FSL were confirmed by observing HIT-T15 pancreaticβ cell growth curve and the influences of HIT-T15 pancreaticβ cell proliferation by different concentrations of glucose and FSL. Afterwards, the influence of FSL on HIT-T15 pancreaticβ cell insulin secretion, the expression of insulin receptor mRNA and insulin receptor substrate (IRS) 1 protein were measured under the environment of high glucose. The results showed that 50 mmol·L-1 glucose can significantly inhibit the proliferation of HIT-T15 pancreaticβ cell (P < 0.01). The 50μg·mL-1 FSL can significantly promote the proliferation of HIT-T15 pancreaticβ cells (P < 0.01), the insulin secretion (P < 0.05), the expression of insulin receptor mRNA (P < 0.05), and the protein expression of IRS 1 (P <0.01). It was concluded thatGuava leaf total flavonoids can promote the insulin secretion of HIT-T15 pancreaticβ cells under the circumstance of high concentration of glucose which may be related to its effect of increasing expression of insulin receptor mRNA and IRS-1 protein.
