Prevention Effect of Danlou Pills on Atherosclerosis through PI3K/AKT Signal Pathways in Rabbit Atherosclerosis Model
10.11842/wst.2015.06.013
- VernacularTitle:丹蒌片通过PI3K/Akt信号通路抗动脉粥样硬化机制研究＊
- Author:
He WANG
;
Minghua LUO
;
Xiaojun SHEN
;
Jinhong XIE
;
Yushan CHEN
;
Chunying SI
;
Liang LI
;
Huaimin GUAN
- Publication Type:Journal Article
- Keywords:
Danlou pills;
atherosclerosis;
PI3K/AKT;
inflammation
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(6):1194-1197
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect ofDanlou pills on prevent atherosclerosis from hypercholesterolemia rabbit and its relationship with inflammatory factors as well as PI3K/AKT signal pathways. A total of 24 Japanese male white rabbits were randomly divided into the control group (CL), model group (M) and Danlou group (DL), with 8 in each group. Normal diet was given to CL rabbits. High-fat diet was given to rabbits in other groups to establish the atherosclerosis model. Danlou pills (0.5 g·kg-1·d-1) were also given to DL rabbits. Rabbits were sacrificed after 9-week medication. The contents of blood lipid, TNF-α and IL-6 were detected. HE staining was used in the observation of histological changes in the aorta. Western blot was used to observe PI3K and p-AKT expression in the aorta. The results showed that compared with CL, the contents of TG, TC, LDL, IL-6 and TNF-α were significantly increased in M (P < 0.01); PI3K and p-AKT expression in the aorta were significantly decreased (P < 0.01). Compared with M, blood lipid, IL-6 and TNF-α were significantly reduced in DL (P < 0.05, orP < 0.01); PI3K and p-AKT expression were significantly increased (P < 0.01). It was concluded thatDanlou pills had prevention effects on atherosclerosis through reducing blood lipid and inflammatory factors. The action mechanism maybe related to the activation of PI3K/AKT signal pathways.
