Regulation Mechanism and Significance of TCMBu-Shen Gu-Jin Prescription on IL-1 and TNF-αof Knee Osteoarthritis
10.11842/wst.2015.06.014
- VernacularTitle:传统中医药补肾固筋方对膝骨性关节炎IL-1、TNF-α的调控机制研究及意义
- Author:
Chong ZHANG
;
Hengshu WANG
;
Lingjiao WANG
;
Chunli ZHANG
;
Yun HUANG
;
Zhongqiu WU
- Publication Type:Journal Article
- Keywords:
Bu-Shen Gu-Jin Prescription;
knee osteoarthritis;
cartilage;
IL-1;
TNF-α
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(6):1198-1202
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the effect of traditional Chinese medicine (TCM)Bu-Shen Gu-Jin Prescription (BSGJP) on the expression and action mechanism of interleukin-1 (IL-1) and tumour necrosis factor-alpha (TNF-α) in knee osteoarthritis (KOA) rabbit model. A total of 76 8-month healthy male New Zealand white rabbits were randomly divided into the control group, model group, TCM BSGJP group and western medicine group, with 19 rabbits in each group. Except the control group, improved Hulth modeling method was used in the animal model establishment. After 8-week consecutive medication, the serum, part of the synovial membrane tissues on the anterior side of the knee joint, articular cartilage and subchondral bone of the medial condyle of the femur were collected. Enzyme-linked immunosorbent assay (ELISA) was used in the content determination of IL-1 and TNF-α. The results showed that the narrowing of the joint space of the western medicine group were in between the model group and the TCM BSGJP group with obvious osteophyte formation. The TCM BSGJP group had slight narrowing of the joint space, mild rough of the articular cartilage surface, and slight osteophyte formation. The contents of IL-1 and TNF-α in the model group were higher than than of the control group (P < 0.01). Both medication groups were lower than the model group (P < 0.01). There was no significant difference between the two medication groups. It was concluded that BSGJP effectively reduced the secretion of IL-1 and TNF-α, delayed the deterioration of cartilage, which provided scientific experimental evidences for the clinical applications.
