Effects of Curcumin Combined with 5-Fluorouracil on Growth Inhibition and Apoptosis Induction in Gastric Cancer Cell Line MGC-803
10.11842/wst.2015.06.015
- VernacularTitle:姜黄素与5-FU联用对胃癌MGC-803细胞的生长抑制与凋亡诱导作用的研究＊
- Author:
Ji LIU
;
Yundan XU
;
Gang ZHAO
- Publication Type:Journal Article
- Keywords:
Curcumin;
5-FU;
MGC-803 cell;
growth inhibition;
apoptosis;
cell cycle
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(6):1203-1209
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to provide experimental evidence for clinical application of curcumin to strengthen the effect of 5-Fluorouracil (5-FU) in gastric cancer treatment, so as to reduce the dose of 5-FU and its side-effect in the future treatment. MTT assay was used to detect medication effect on MGC-803 cell growth inhibition; AO/EB double staining fluorescence microscopic observation and FITC/PI double staining flow cytometry were used to detect medication effect on cell apoptosis. Flow cytometry was based on PI staining used to detect medication effect on MGC-803 cell cycle. The results showed that curcumin (25μmol·L-1) with low-dose (2.4μmol·L-1) and middle-dose (4.8μmol·L-1) 5-FU can effectively inhibit MGC-803 cell growth, induce cell apoptosis and block cell cycle in S phase. Which showed dose-time dependent manner. The combined use of curcumin with low-dose 5-FU was more effective than the middle-dose (4.8μmol·L-1) 5-FU alone (P < 0.01); similarly, the combined use of curcumin with middle-dose 5-FU was more effective than the use of high-dose (9.6μmol·L-1) 5-FU alone (P < 0.01). It indicated that curcumin can enhance the antitumor effect of 5-FU against MGC-803 cells in a dose-time dependent manner. It was concluded that the study provided a preliminary experimental basis for using curcumin as an adjuvant to 5-FU with the benefit of reducing its dose and toxicity in the clinical treatment of gastric cancer.
