Bronchoalveolar lavage fluid mycoplasm load and the immune response of Th1/Th2 cells in children withMycoplas-ma pneumoniae pneumonia
10.3969/j.issn.1000-3606.2015.08.001
- VernacularTitle:肺炎支原体肺炎患儿肺泡灌洗液病菌量及其与Th1/Th2细胞免疫应答的关系
- Author:
Jing LI
;
Jun BAO
;
Yixiao BAO
;
Quanhua LIU
;
Ruoxu JI
;
Qian LIN
- Publication Type:Journal Article
- Keywords:
Mycoplasma pneumoniae pneumonia;
Th1/Th2 immune balance;
bronchoalveolar lavage lfuid;
child
- From:
Journal of Clinical Pediatrics
2015;(8):681-685
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo understand the relationship between mycoplasm load in bronchoalveolar lavage lfuid (BALF) with the status of Th1/Th2 immune response in children withMycoplasma pneumoniae pneumonia (MPP).MethodsThe levels of IL-4, IFN, IL-8 , TNF-α in BLAF and total IgE, ECP in serum from 90 children with MPP were measured by ELISA.MP DNA in BALF was detected quantitatively by lfuorescent real-time PCR. Children with MPP (n=90) were divided into two groups of low MP-DNA load (n=24) and high MP-DNA load (n=26) according to the copies of MP DNA in BALF. The cytokines in BALF, and total IgE and ECP in serum were compared between the two groups. The relationship between the levels of cytokines in BLAF and the copies was evaluated.ResultsThe levels of IL-4 and the IL-4/IFN ratio in BALF from the high DNA-load group were signiifcantly higher than that of the low group (t=4.280, 2.076, allP<0.05). The level of IL-4 was signiifcantly correlated with the copies of MP-DNA in BALF from children with MPP (r=0.509,P<0.05). The percentage of total IgE and ECP positive result in serum from the high DNA-load group is higher than that of the low group. (χ2=24.638, 6.392,allP<0.05).Conclusion Infection with high-load MP in children may cause the imbalance of Th1/Th2. And the Th2 cytokines response seems predomi-nant.
