Studies on HPLC Fingerprint Difference ofRadix Platycodonis from Different Origins Based on Platycodins
10.11842/wst.2015.05.015
- VernacularTitle:不同产地桔梗皂苷成分HPLC指纹图谱比较研究
- Author:
Jinxiang ZENG
;
Xiangxiang FANG
;
Jixiao ZHU
;
Bo WU
;
Guoyue ZHONG
;
Fuqing LIU
;
Hongze LI
;
Fengyu HAN
- Publication Type:Journal Article
- Keywords:
Radix Platycodonis;
fingerprint;
platycodins;
quality differences
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(5):1000-1006
- CountryChina
- Language:Chinese
-
Abstract:
The HPLC fingerprint differences ofRadix Platycodonis from different origins were studied to provide references for their quality control and production. The total platycodins were purified by DB101 macroporous resin. HPLC-ELSD fingerprints of the total platycodins for 39 batches ofRadix Platycodonis samples from 9 provinces were performed on an Agilent HC-C8 column (4.6 mm x 250 mm, 5μm) with gradient elution. The mobile phase was acetonitrile-0.5% acetic acid. The injection volume was 6μL. The flow rate was 0.5 mL·min-1. The temperature of drift tube was set at 90℃. And the gas flow (N2) was set at 1.2 mL·min-1. The results showed that there were large differences in the quality ofRadix Platycodonis from different origins with the common fingerprints of 6 batches of samples fromChifeng in Inner Mongolia as references. The quality ofRadix Platycodonis was closely related to the seeds, the ecological environment, the way of drying and storing and so on. It was concluded that it was important to strengthen the provenance base construction, standardization of the seeds, reasonable formulation of the regionalization, and standardization of the production processing for the cultivation and production ofRadix Platycodonis.
