Detection ofN-myc andALK abnormality in and clinicopathological analyses of 83 cases of neuroblastoma
10.3969/j.issn.1000-3606.2015.08.010
- VernacularTitle:83例神经母细胞瘤患儿N-myc和ALK基因检测及临床病理分析
- Author:
Weiya WANG
;
Jinnan LI
;
Weilu WU
;
Min CHEN
;
Gandi LI
;
Zhigui MA
- Publication Type:Journal Article
- Keywords:
neuroblastoma;
N-mycgene;
ALKgene;
clinicopathological analysis
- From:
Journal of Clinical Pediatrics
2015;(8):720-725
- CountryChina
- Language:Chinese
-
Abstract:
ObjectivesTo retrospectively analyze the clinicopathological features of neuroblastoma (NB) and investigate the signiifcance of abnormality ofN-myc and anaplastic lymphoma kinase (ALK) gene copy number change as well asALKmu-tations in NB.Methods Eighty-three NB patients were collected and classiifed into different subgroups according to the clinical stage and histology. Fluorescence in situ hybridization (FISH) was performed to detect the abnormalities ofN-mycandALK genes. The extracted DNA was ampliifed by PCR and sequenced to investigate the point mutations of theALK gene. Follow-up data were collected and survival analysis was performed.ResultsFISH detection showed that the aberration ofN-mycgene copy number presented as gain and ampliifcation. The aberration ofALK gene presented as point mutation and gain. It was shown that 17 cases had the abnormality of bothN-myc andALK gene. Survival analysis showed that the prognostic factors included the clinical stage, age and abnormality ofN-myc genes.ConclusionDetection ofN-myc andALK abnormality in NB would be helpful for evaluating the prognosis and providing theoretical basis forALK target therapy.
